ABSTRACT
Objective To explore the feasibility of the Coapresta2000 automatic coagulation analyzer for detecting C‐reactive protein(CRP) .Methods The performance of the Coapresta2000 automatic coagulation analyzer for detecting CRP was analyzed ,in‐cluding accuracy ,linearity ,precision ,carryover rate and the correlation between the Ottoman automatic special protein real time ana‐lyzer and the Coapresta2000 automatic coagulation analyzer .Results The men recovery rate of 3 batches of reagent by the Coapres‐ta2000 automatic coagulation analyzer for detecting CRP for detecting plasma CRP was 106 .0% with better accuracy ;the within‐run precision was less than 10% and the inter‐day precision was less than 15% ;the carryover rate was 0 .32% ;the related equation in the Ottoman automatic special protein real time analyzer and the Coapresta2000 automatic coagulation analyzer for detecting plas‐ma CRP was Y=0 .001 2X+0 .489 ,the correlation coefficient was r2 =0 .997 5 ,the difference was not statistically significant(P>0 .05) .The CRP results of plasma sample detected by these two instruments was highly correlative ;the linearity in the Coapres‐ta2000 automatic coagulation analyzer for detecting CRP was 0-200 mg/L .Conclusion The Coapresta2000 automatic coagulation analyzer for detecting CRP has better within‐run precision ,low carryover rate ,high correlation with the Ottoman automatic special protein real time analyze ,which has a value for further study .
ABSTRACT
Objective To investigate the effect of anterior cervical discectomy and interbody fusion ( ACDF) combined with titanium plate fixation technique and subtotal vertebrectomy and fusion and titanium plate internal fixation operation ( ACCF) on adjacent segmental cervical spondylotic myelopathy. Methods 76 patients with double segment spondylotic cervical myelopathy were randomly divided into AC-CF group and ACDF group,the relevant indicators of operation period,spinal cord function,anteroposterior and lateral of cervical spine and flexion-extension dynamic radiographs were observed,the Cobb’ s angle and fusion segmental height and bone fusion were recorded. Results The operation related index results showed that the operation time,bleeding volume and hospitalization time of patients in ACDF were less than those in ACCF group (P 0. 05). The postoperative Cobb’s angle degree of ACDF group is better than that of AC-CF group (P<0. 05). Conclusion The effect of ACCF and ACCF on the treatment of two-level CSM were satisfactory,of which ACDF has the advantages of short operation time,less bleeding,fusion segments of Cobb’ s angle and fewer complications.
ABSTRACT
AIM: To observe the effect of Qingluo Tongbi Granule(QLTBG)——containing serum on the proliferation of osteoblasts(OB) and osteoclasts(OC),the activity of AKP of OB. METHODS: (1) OB were separated from the skull of SD rats aged 1 d and OC from the thighbone and shinbone of SD rats aged 5 d.(2) The proliferation of OB and OC was detected by MTT method,and the activity of AKP of OB was examined by diazol method. RESULTS: The proliferation capability of OB was strengthened markedly by the rat sera containing 7.2,14.4 g/kg QLTBG,which could reinforce the activity of AKP obviously as well,while the proliferation of OB was inhibited remarkably by the rat sera containing 3.6,7.2,14.4 g/kg QLT. CONCLUSION: The sera containing QLTBG could enhance the proliferation of OB and the activity of AKP,restrain the proliferation of OC simultaneously.